Tammi Taylor
Jackson State University, USATitle: Neuroprotective and regenerative roles of intranasal Wnt-3a Administration after focal ischemic stroke in mice
Abstract
Wnt signaling is a conserved pathway involved in expansion of neural
progenitors and lineage specification during development. However, the role of
Wnt signaling in the post-stroke brain has not been well-elucidated. We
hypothesized that Wnt-3a would play an important role for neurogenesis and
brain repair. Adult male mice were subjected to a focal ischemic stroke
targeting the sensorimotor cortex. Mice that received Wnt-3a (2?µg/kg/day, 1?h
after stroke and once a day for the next 2 days, intranasal delivery) had
reduced infarct volume compared to stroke controls. Wnt-3a intranasal treatment
of seven days upregulated the expression of brain-derived growth factor (BDNF),
increased the proliferation and migration of neuroblasts from the
subventricular zone (SVZ), resulting in increased numbers of newly formed
neurons and endothelial cells in the peri-infarct zone. Both the molecular and
cellular effects of Wnt-3a were blocked by the Wnt specific inhibitors XAV-939
or Dkk-1. In functional assays, Wnt-3a treatment enhanced the local cerebral
blood flow (LCBF) in the peri-infarct, as well as improved sensorimotor
functions in a battery of behavioral tests. Together, our data demonstrates
that the Wnt-3a signaling can act as a dual neuroprotective and regenerative factor
for the treatment of ischemic stroke.
Key words: Stroke, Wnt3a, neuroblast,
proliferation,
Biography
Dr. Taylor joined the faculty as an Assistant Professor of Genetics in the Department of Biology in the College of Science, Engineering, and Technology at Jackson State University. While at JSU, Dr. Taylor is a Research Center for Minority Investigators (RCMI) faculty member, a graduate and undergraduate student advisor, mentor, and professor. She obtained her PhD in Microbiology from Indiana University, a Masters of Science degree in Biology, from Jackson State University, and her Bachelors of Science degree in Biology from Tougaloo College. She was a Fellowship in Research and Science Teaching (FIRST) postdoctoral fellowship at Emory University in the Departments of Anesthesiology and Neurology. Her current research focuses on determining if there are stroke risk factor differences in African American men and women Jackson Heart Study patients 2001-2016.
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